Journal: Lab on a Chip
Article Title: Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes
doi: 10.1039/d2lc00245k
Figure Lengend Snippet: Parallel formation and cultivation of human adipose tissue-derived adult stem cells in 3D on-chip. A) Timeline of hASC self-aggregation in the cell culture chamber. Scale bar: 250 μm. B) Intrachip variability of the equivalent diameter of the 3D cell cultures. n = 6 biological repeats. C) Interchip variability of the equivalent diameter of formed hASC 3D cell cultures. n = 96 technical replicates per chip, all comparisons exhibited a p < 0.05 (one-way ANOVA) except for n.s. = not significant. D) Equivalent diameter of the hASC 3D cell cultures during on-chip culture. n = 4–12 technical replicates. E) Rates of successful retrieval of the hASC 3D cell cultures from the cell culture chambers. n = 3 biological replicates, * p < 0.05 (one-way ANOVA).
Article Snippet: Human adipose tissue-derived stem cells (hASCs; ASC-F-ZB) were purchased from ZenBio (NC, USA) and maintained in routine 2D cell culture using subcutaneous preadipocyte growth medium (PM-1; ZenBio) according to the supplier's protocol. hASCs at passages 6–8 were used for the experiments.
Techniques: Derivative Assay, Cell Culture